La Folie, Quo Vadis, Evo-Devo?

http://www.jbc.org/cgi/content/full/283/5/2495
Haanstra et al. 283 (5): 2495 -- Journal of Biological Chemistry
Westerhoff group出來的作品, modeling Isoenzymes of phosphoglycerate kinase in Trypanosoma brucei兩個stage的變化
這篇顯示出這大頭調教出的學生, 思考的完整性與作品呈現的風格值得學習.
雖說還是玩metabolic pathway這老方法, 但是並不只侷限於flux analysis, 而是更上一層模擬其細微的控制
四個重要的factors(or variables): 1. mRNA abundance 2. RNA precursor stability 3. trans splicing 4. ribosome loading
層級跨越post-transcription與translation
把這四個要素寫成model然後進行模擬, 並下了幾個結論:
1. Regulation of mRNA levels does not occur by transcription, but by adjusting mRNA degradation.
2. The contribution of splicing to regulation is negligible
3. All cases where splicing is faster than RNA precursor degradation